NR 105.05(1)(a)1.1. At least one is a salmonid fish in the family Salmonidae in the class Osteichthyes,
NR 105.05(1)(a)2.2. At least one is a non-salmonid fish from another family in the class Osteichthyes, preferably a commercially or recreationally important warmwater species,
NR 105.05(1)(a)3.3. At least one is a planktonic crustacean (e.g., cladoceran, copepod),
NR 105.05(1)(a)4.4. At least one is a benthic crustacean (e.g., ostracod, isopod, amphipod, crayfish),
NR 105.05(1)(a)5.5. At least one is an insect (e.g., mayfly, dragonfly, damselfly, stonefly, caddisfly, mosquito, midge),
NR 105.05(1)(a)6.6. At least one is a fish or amphibian from a family in the phylum Chordata not already represented in one of the other subdivisions.
NR 105.05(1)(a)7.7. At least one is an organism from a family in a phylum other than Arthropoda or Chordata (e.g., Rotifera, Annelida, Mollusca), and
NR 105.05(1)(a)8.8. At least one is an organism from a family in any order of insect or any other phylum not already represented in subds. 1. to 7.
NR 105.05(1)(a)9.9. If all 8 of the families in subds. 1. to 8. are represented, an acute toxicity criterion may be developed for surface waters classified as cold water using information on all of those families. If an acute toxicity criterion is developed for surface waters classified as cold water, acute toxicity criteria may also be developed for any of the surface water classifications in s. NR 102.04 (3) (b) to (e) using the procedure in sub. (2) or (3) and data on families in subds. 1. to 8. which are representative of the aquatic life communities associated with those classifications. For each substance, in no case may the criterion for a lower quality fish and aquatic life subcategory as defined in s. NR 102.04 be less than the criterion for a higher quality fish and aquatic life subcategory.
NR 105.05(1)(a)10.10. For a substance, if all of the families in subds. 1. to 8. are not represented, an acute toxicity criterion may not be developed for that substance. Instead, any available data may be used to develop a secondary acute value (SAV) for that substance according to s. NR 105.02 (3) and sub.(4).
NR 105.05(1)(b)(b) The acceptability of acute toxicity test results shall be judged according to the guidelines in section IV of the United States environmental protection agency’s 1985 “Guidelines for Deriving National Numerical Water Quality Criteria for the Protection of Aquatic Organisms and Their Uses” or 40 CFR Part 132, Appendix A. II, IV and V, as stated on September 1, 1997, is incorporated by reference.
NR 105.05 NoteNote: Copies of 40 CFR Part 132, Appendix A Sections II, IV and V are available for inspection in the offices of the department of natural resources, secretary of state and the legislative reference bureau, Madison, WI or may be purchased from the superintendent of documents, US government printing office, Washington, D.C. 20402.
NR 105.05(2)(2)Acute toxicity criteria for substances with toxicity unrelated to water quality parameters. If the acute toxicity of a substance has not been adequately shown to be related to a water quality parameter (i.e., hardness, pH, temperature, etc.), the acute toxicity criterion (ATC) is calculated using the procedures specified in this subsection.
NR 105.05(2)(a)(a)
NR 105.05(2)(a)1.1. For each species for which at least one acute value is available, the species mean acute value (SMAV) is calculated as the geometric mean of all acceptable acute toxicity tests using the guidelines in sub. (1) (b).
NR 105.05(2)(a)2.2. For each genus for which one or more SMAVs are available, the genus mean acute value (GMAV) is calculated as the geometric mean of the SMAVs available for the genus.
NR 105.05(2)(b)(b) The GMAVs are ordered from high to low.
NR 105.05(2)(c)(c) Ranks (R) are assigned to the GMAVs from 1 for the lowest to N for the highest. If 2 or more GMAVs are identical, successive ranks are arbitrarily assigned.
NR 105.05(2)(d)(d) The cumulative probability (P) is calculated for each GMAVs as P=R/(N + 1).
NR 105.05(2)(e)(e) The 4 GMAVs are selected which have P closest to 0.05. If there are less than 59 GMAVs, these will always be the lowest GMAVs.
NR 105.05(2)(f)(f) Using the selected GMAVs and Ps, the ATC is calculated using the following:
NR 105.05(2)(f)1.1. Let EV = sum of the 4 ln GMAVs,
      EW = sum of the 4 squares of the ln GMAVs,
      EP = sum of the 4 P values,
      EPR = sum of the 4 square roots of P, and
      JR = square root of 0.05.
NR 105.05(2)(f)2.2. S = ((EW - (EV)2 /4)/(EP-(EPR)2 /4))0.5.
NR 105.05(2)(f)3.3. L = (EV - S(EPR))/4.
NR 105.05(2)(f)4.4. A = (JR)(S) + L.
NR 105.05(2)(f)5.5. Final Acute Value (FAV)= eA.
NR 105.05(2)(f)6.6. ATC = FAV/2.
NR 105.05(2)(g)(g) If, for a commercially, recreationally or ecologically important species, the geometric mean of the acute values from flow-through tests in which the concentration of test material was measured is lower than the calculated ATC [FAV], then that geometric mean is used as the ATC [FAV] instead of the calculated one.
NR 105.05(2)(h)(h) Table 1 contains the acute toxicity criteria for fish and aquatic life subcategories listed in s. NR 102.04 (3) that are calculated using the procedures described in this subsection for substances meeting the database requirements indicated in sub. (1) (a).