Data assessment and acceptance criteria for quality control measures.
Corrective actions and contingencies for handling out of control or unacceptable data.
NR 149.40 History
History: CR 17-046: cr. Register February 2021 No. 782, eff. 6-29-21. NR 149.41(1)(1)
The laboratory shall use methods for environmental testing required by covered programs under this chapter and that are suitable for the matrix, type of analyte, expected level of analyte, regulatory limit, and potential interferences in the samples to be tested.
NR 149.41 Note
Sources, including the following as updated, likely contain methods that are acceptable for testing under this chapter: The EPA, the department, Standard Methods for the Examination of Water and Wastewater, Test Methods for Evaluating Solid Waste, Physical/Chemical Methods – SW-846, American Society for Testing and Materials, and the U.S. Geological Survey Agency.
When methods are not specified by covered programs under this chapter or specified in permits issued by the department, the laboratory shall consult with the department to select a method that meets the requirements in sub. (1)
When using methods associated with the methods compendium document, “Test Methods for Evaluating Solid Waste,” the laboratory shall comply with the minimum requirements of the methods as written and state which options are being implemented when options exist.
NR 149.41 Note
The documents above can be found on the Wisconsin department of natural resources laboratory accreditation program website and are available for inspection at the offices of the department and the legislative reference bureau.
The department will assess the scientific validity of method modifications to determine if the modification is within the scope of a method.
NR 149.41 History
History: CR 17-046: cr. Register February 2021 No. 782, eff. 6-29-21. NR 149.42(1)(1)
The department may allow the use of alternative methods from those required by covered programs, including the safe drinking water program, if a laboratory requests approval and if the EPA has granted approval for the alternative methods.
On a case-by-case basis, the department may allow the use of methods other than those required by covered programs for any of the following situations:
After consultation with the department, the manager of a covered program determines that the allowance does not result in a detrimental effect on the quality and defensibility of the results to be generated.
The request is for approval of a method that employs a new or emerging technology and there is documentation that substantiates the validity of the new or emerging technology for the intended purpose.
Requests to use an alternative method shall include the reason for seeking the approval, a description of the principles of any new or emerging technology involved, and the potential scope of application of the method. The department may establish criteria for validating the method for the specific application and scope requested. If the laboratory's method validation results meet the established validation criteria, the department shall allow the use of the method for the specific application and scope requested.
The department shall approve or deny the request for consideration of approval for use within 90 days from the receipt of the request. The department shall consider in its decision whether the covered programs that would be the recipients of the data generated have a demonstrated need for allowing the alternative method.
The department may charge a fee under s. 299.11 (5) (d)
, Stats., if it is necessary to verify the results of any validation data submitted by a laboratory requesting use of an alternative method.
NR 149.42 History
History: CR 17-046: cr. Register February 2021 No. 782, eff. 6-29-21. NR 149.43(1)(ag)
The laboratory shall ensure that the environmental conditions of its facility do not adversely affect the required quality of any measurement.
Laboratory facilities shall ensure effective separation between neighboring areas in which incompatible analytical activities take place. The laboratory shall take measures to prevent cross-contamination.
Access to and use of areas affecting the quality of environmental tests shall be controlled to an extent commensurate with the type of analysis and samples analyzed by a laboratory.
The laboratory shall monitor, control, and record environmental conditions when the environmental conditions are required by the methods or when the environmental conditions influence the quality of test results.
NR 149.43 History
History: CR 17-046: cr. Register February 2021 No. 782, eff. 6-29-21; renum. (1) (intro.), (a) to (1) (ag), (ar) under s. 13.92 (4) (b) 1., Stats., Register February 2021 No. 782. NR 149.44(1)(a)(a)
The laboratory shall furnish the equipment necessary and required for the correct performance of all the environmental tests and associated preparations and activities it performs.
The laboratory shall use equipment and software for testing and calibration that achieves the accuracy required to comply with the requirements of the methods or specifications relevant to the environmental testing performed by the laboratory.
The laboratory shall use support equipment only for its intended purpose, and it shall keep that equipment in working order by routine and preventive maintenance.
When support equipment leaves the direct control of the laboratory for maintenance or for any other reason, the laboratory shall ensure that the function and calibration status of that equipment is checked or demonstrated to be in working order before the equipment is returned to service.
Calibration and verification of support equipment. NR 149.44(3)(a)(a)
The laboratory shall calibrate or verify all support equipment within that equipment's range of use using available reference materials traceable to NIST. When reference materials traceable to NIST are not commercially available, the laboratory shall use materials of a quality that will ensure the accuracy of the calibrated or verified support equipment for its intended use.
The acceptability criteria for these calibration or verification checks shall be established by the methods, or in the absence, department guidance.
NR 149.44 Note
Department guidance can be found on the Wisconsin department of natural resources laboratory accreditation program website.
The laboratory shall establish a procedure for calibrating or verifying the calibration of support equipment which shall include all the following elements:
Procedures for utilization of correction factors when there is a bias.
Evaluation criteria used which defensibly documents the continued accuracy of the equipment.
Procedures for addressing equipment which fails to meet calibration or verification requirements.
Minimum verification frequencies include all the following:
Annually: devices used to measure atmospheric pressure and temperature.
Quarterly: mechanical and automatic volumetric dispensing devices, including pipettes.
Monthly: balances, with one weight in the expected range of use. Balance weights shall be all the following:
Traceable to NIST and of class 2 quality or better. Certified for accuracy every five years by a metrology service outside the laboratory. Alternatively, new weights of class 2 quality or better, traceable to NIST, shall be purchased for use. Weight recertification shall be performed sooner than every five years if balance checks performed using these weights suggest that a change in the certified weights has occurred.
NR 149.44 Note
Weights that are currently NIST traceable may be used to verify other weights.
Each day of use: when specific temperatures are required by method, regulation, or covered program, the operating temperature of the equipment used to control temperatures shall be checked and documented.
All the following are exempt from accuracy verification under this section:
The laboratory shall use personnel properly trained to operate analytical instruments. Instructions on the use and maintenance of equipment shall be available to instrument operators.
The laboratory shall properly maintain, inspect, and clean all instruments. The laboratory shall establish procedures for the maintenance of analytical instruments to prevent contamination or deterioration that may affect reported results.
The laboratory shall remove from service all analytical instruments that give suspect results or that have been shown to be defective or outside of performance specifications.
When analytical instruments leave the direct control of the laboratory for maintenance or for any other reason, the laboratory shall ensure that the instruments are functional and that a new initial calibration has passed to demonstrate that the instruments are in satisfactory working order before returned to service.
NR 149.44 History
History: CR 17-046: cr. Register February 2021 No. 782, eff. 6-29-21. NR 149.442(1)(1)
Sample acceptance policy and sample handling procedures. NR 149.442(1)(a)
The laboratory shall have and follow a written policy that clearly outlines the conditions under which samples will be accepted or rejected for analysis or under which associated reported results will be qualified.
NR 149.442 Note
Section NR 149.47 (4)
provides rejection criteria.
The laboratory shall receive drinking water samples in a secure manner so that the integrity of the sample is maintained.
When samples received do not conform to the descriptions provided by a collector or do not conform to sample acceptance requirements, the laboratory shall consult with the collector or client to determine the proper processing or disposition of the samples.
The laboratory shall place a unique identification code on a sample container as a durable label. The unique identification code shall be used as a link to associate samples with the complete sample history, including treatment and analysis, while in the laboratory's possession.
A laboratory shall follow the sample preservation procedures and holding times required by state and federal regulations.
NR 149.442 Note
Sample preservation procedures and holding times are given in 40 CFR Part 136
, 40 CFR Part 141
, NR 219, SW-846 “Test Methods for Evaluating Solid Waste” and may be specified in the methods.
NR 149.442 Note
Links to 40 CFR Part 136
, 40 CFR Part 141
, NR 219, and SW-846 can be found on the Wisconsin department of natural resources laboratory accreditation program website.
The laboratory shall measure and document the sample temperature at the time of receipt when temperature preservation is required.
The laboratory shall consider any sample requiring preservation at ≤
6 ºC to be preserved if the sample is received at a temperature greater than its freezing point to 6 ºC. When samples are received on the same day that they were collected, the samples may not yet have reached the appropriate temperature by the time they arrived at the laboratory. These samples may be considered acceptable, without the need to qualify the data, if all the following apply:
Samples were received at the laboratory on ice. “Blue ice” packs may not be considered as received on ice.
When sample temperature measurements are required, the laboratory shall record any of the following:
The laboratory shall verify the pH of each bottle received for samples requiring chemical preservation to a specific pH requirement under this section. Bottles not received at the proper pH may be adjusted at the laboratory provided that the methods allow preservation upon receipt and the lab retains documentation of its actions.
NR 149.442 Note
pH verification is only required from the bottle that is analyzed.
Sample receipt documentation.
The laboratory shall document the receipt and condition of all samples in chronological hard copy or electronic records. The records may be maintained in any format that retains all the following information:
The identity of the client or entity submitting samples, or the project associated with the received samples.
The times of sample collection for samples to be analyzed for tests with holding times expressed in hours.
The unique sample identification code assigned by the laboratory.